Effects of Quercetin on Tubular Cell Apoptosis and Kidney Damage in Rats Induced by Titanium Dioxide Nanoparticles|
Alidadi, Hadis; Khorsandi, Layasadat & Shirani, Maryam
Background: Recent studies have demonstrated that many nanoparticles have an adverse
or toxic effect on the kidney.
Objective: To investigate the nephroprotective effect of quercetin (QT) against renal injury
induced by titanium dioxide nanoparticles (NTiO2) in rats.
Methods: NTiO2-intoxicated rats received 50 mg/kg of NTiO2 for seven days. The QT +
NTiO2 group was pretreated with QT for seven days before being administered NTiO2. Uric acid,
creatinine, and blood urea nitrogen were considered to be biomarkers of nephrotoxicity. Catalase
(CAT) and superoxide dismutase (SOD) activities and renal levels of malondialdehyde (MDA) were
measured to assess the oxidative stress caused by NTiO2.
Results: NTiO2 significantly increased the plasma level of the biomarkers. It also
significantly decreased the activities of CAT (P = 0.008) and SOD (P = 0.004), and significantly
increased the MDA levels (P = 0.007). NTiO2 caused proximal tubule damage, the accumulation
of red blood cells, the infiltration of inflammatory cells, and reduced the glomerular diameters,
as well as induced apoptosis in the proximal tubules. Pre-treatment with QT attenuated the
histological changes, normalised the plasma biomarkers, suppressed oxidative stress, ameliorated
the activities of CAT (P = 0.007) and SOD (P = 0.006), and reduced apoptosis (P < 0.001).
Conclusion: QT was found to have a potent protective effect against nephrotoxicity
induced by NTiO2 in rats. It also reduced apoptosis caused by NTiO2.
nanoparticles; quercetin; nephrotoxicity; oxidative stress; anti-oxidants; rats