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Trkb-IP3 Pathway Mediating Neuroprotection in Rat Hippocampal Neuronal Cell Culture Following Induction of Kainic Acid
Chong, Pei Nei; Sangu, Muthuraju; Huat, Tee Jong; Reza, Faruque; Begum, Tahamina; Mohamed Yusoff, Abdul Aziz; Jaafar, Hasnan & Abdullah, Jafri Malin
Abstract
Background: Following brain injury, development of hippocampal sclerosis often led to
the temporal lobe epilepsy which is sometimes resistant to common anti-epileptic drugs. Cellular
and molecular changes underlying epileptogenesis in animal models were studied, however, the
underlying mechanisms of kainic acid (KA) mediated neuronal damage in rat hippocampal neuron
cell culture alone has not been elucidated yet.
Methods: Embryonic day 18 (E-18) rat hippocampus neurons were cultured with poly-L-lysine coated glass coverslips. Following optimisation, KA (0.5 μM), a chemoconvulsant agent,
was administered at three different time-points (30, 60 and 90 min) to induce seizure in rat
hippocampal neuronal cell culture. We examined cell viability, neurite outgrowth density and
immunoreactivity of the hippocampus neuron culture by measuring brain derived neurotrophic
factor (BDNF), γ-amino butyric acid A (GABAA) subunit α-1 (GABRA1), tyrosine receptor kinase B
(TrkB), and inositol trisphosphate receptor (IP3R/IP3) levels.
Results: The results revealed significantly decreased and increased immunoreactivity
changes in TrkB (a BDNF receptor) and IP3R, respectively, at 60 min time point.
Conclusion: The current findings suggest that TrkB and IP3 could have a neuroprotective
role which could be a potential pharmacological target for anti-epilepsy drugs.
Keywords
rat hippocampal neuron culture; IP3 & BDNF-TrkB receptor; GABAA receptor; kainic acid; epilepsy
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