Malawi Medical Journal
College of Medicine, University of Malawi and Medical Association of Malawi
Vol. 23, No. 4, 2015, pp. 105-109
Bioline Code: mm11028
Full paper language: English
Document type: Research Article
Document available free of charge
Malawi Medical Journal, Vol. 23, No. 4, 2015, pp. 105-109
© Copyright 2011 - Malawi Medical Journal
Cytosine Arabinoside reduces the numbers of granulocyte macrophage colony forming cells (GMCFC) and high proliferative potential colony forming cells (HPP-CFC) in vivo in mice|
Teleka, Stanley; Chijuwa, Alexander; Senga, Edward & Chisi, John E.
Cytosine arabinoside (Ara-C) is an S-phase specific cytotoxic
drug used in the treatment of malignancies. It is converted to Cytosine
Arabinoside triphosphate (Ara-CTP) in the cell. Cytosine Arabinoside
triphosphate, reversibly displaces deoxy cytidine triphosphate from DNA
polymerase for incorporation into DNA. This process leads to cell death.
To investigate the in vivo effects of Ara-C on the Granulocyte
Macrophage Colony Forming Cells (GM-CFC) and High Proliferative
Potential Colony Forming Cells (HPP-CFC) respectively in mice.
Ara-C (150mg/kg) was administered intraperitoneally (i.p)
once to mice and bone marrow cells sampled on days 1, 3 and 6.
Ara-C reduced the numbers of both GM-CFC and HPP-CFC
in the bone marrow. HPP-CFCs were initially more sensitive to Ara-C
treatment than GM-CFCs. In the six days after treatment the effect on
GM-CFC persisted, while there was a partial recovery in the number of
It is possible that Ara-C disturbs the stem cells niche by
damaging the stromal cells of the bone marrow microenvironment. This
would result in derangement of HPP-CFC proliferation.
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