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Malawi Medical Journal
College of Medicine, University of Malawi and Medical Association of Malawi
ISSN: 1995-7262
Vol. 23, No. 4, 2015, pp. 105-109
Bioline Code: mm11028
Full paper language: English
Document type: Research Article
Document available free of charge

Malawi Medical Journal, Vol. 23, No. 4, 2015, pp. 105-109

 en Cytosine Arabinoside reduces the numbers of granulocyte macrophage colony forming cells (GMCFC) and high proliferative potential colony forming cells (HPP-CFC) in vivo in mice
Teleka, Stanley; Chijuwa, Alexander; Senga, Edward & Chisi, John E.

Abstract

Background:
Cytosine arabinoside (Ara-C) is an S-phase specific cytotoxic drug used in the treatment of malignancies. It is converted to Cytosine Arabinoside triphosphate (Ara-CTP) in the cell. Cytosine Arabinoside triphosphate, reversibly displaces deoxy cytidine triphosphate from DNA polymerase for incorporation into DNA. This process leads to cell death.
Objective:
To investigate the in vivo effects of Ara-C on the Granulocyte Macrophage Colony Forming Cells (GM-CFC) and High Proliferative Potential Colony Forming Cells (HPP-CFC) respectively in mice.
Methodology:
Ara-C (150mg/kg) was administered intraperitoneally (i.p) once to mice and bone marrow cells sampled on days 1, 3 and 6.
Results:
Ara-C reduced the numbers of both GM-CFC and HPP-CFC in the bone marrow. HPP-CFCs were initially more sensitive to Ara-C treatment than GM-CFCs. In the six days after treatment the effect on GM-CFC persisted, while there was a partial recovery in the number of HPP-CFCs.
Conclusion:
It is possible that Ara-C disturbs the stem cells niche by damaging the stromal cells of the bone marrow microenvironment. This would result in derangement of HPP-CFC proliferation.

 
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