Risk Factors for Neonatal Sepsis and Method for Reduction of Blood Culture Contamination|
Krajčinović, S. S.; Doronjski, A.; Barišić, N. & Stojanović, V.
False-positive blood cultures findings may lead to a falsely increased
morbidity and increased hospital costs.
The survey was conducted as retrospective - prospective study and included
239 preterm infants (born before 37 weeks of gestation) who were treated
in Neonatal Intensive Care Unit (NICU) in Institute for Child and Youth
Health Care of Vojvodina during one year (January 1st, 2012 to December
31st, 2012). The retrospective part of the study focused on examination
of incidence of neonatal sepsis and determination of risk factors. In the
prospective part of the study infants were sub-divided into two groups:
Group 1- infants hospitalized in NICU during the first 6 months of the
study; blood cultures were taken by the ‘’clean technique’’ and checklists
for this procedure were not taken. Group 2- neonates hospitalized in
NICU during last 6 months of the study; blood cultures were taken by
‘’sterile technique’’ and checklists for this procedure were taken.
The main risk factors for sepsis were prelabor rupture of membranes, low
gestational age, low birth weight, mechanical ventilation, umbilical venous
catheter placement, and abdominal drainage. Staphylococcus aureus and
coagulase negative Staphylococcus were the most frequently isolated
microorganisms in false-positive blood samples.
Education of employees, use of checklists and sterile sets for blood
sampling, permanent control of false positive blood cultures, as well as
regular and routine monthly reports are crucial for successful reduction
of contamination rates.