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Malawi Medical Journal
College of Medicine, University of Malawi and Medical Association of Malawi
ISSN: 1995-7262
Vol. 30, No. 4, 2018, pp. 243-249
Bioline Code: mm18049
Full paper language: English
Document type: Research Article
Document available free of charge

Malawi Medical Journal, Vol. 30, No. 4, 2018, pp. 243-249

 en Biofilm forming capacity and antibiotic susceptibility of Staphylococcus check for this species in other resources spp. with the icaA/icaD/bap genotype isolated from ocular surface of patients with diabetes
Kıvanç, Sertaç Argun; Arık, Gizem; Akova-Budak, Berna & Kıvanç, Merih


Bacterial biofilm is an exopolysaccharide matrix that is produced by bacteria while they adhere on abiotic or biotic surfaces. The bacteria living in this matrix are more resistant to antibiotics than planctonic bacteria. The biofilm formation property of the bacteria is determined by genes; and this is related to virulence of the microorganism. In ophthalmology, biofilms form especially on abiotic surfaces such as silicon tubes, contact lenses, intraocular lenses etc.
Our aim was to investigate genotypic and phenotypic structures of biofilms that are produced by Staphylococcus spp., which was obtained from the eyes of diabetic patients and determine the effect on antibiotic susceptibility.
The study group was comprised with 83 isolates from diabetic patients and 21 isolates from non-diabetic patients. Presumptive isolates were detected and confirmed by a microbial identification system VITEK II. Automated EcoRI Ribotyping was performed. Biofilm production was detected by Congo Red Agar Plate and Microtiter Plate Assay. Disc diffusion method was used for determination of antibiotic susceptibility of isolates.
Out of the 83 isolates from diabetic patients, 25 were weakly (30%), 20 were moderately (24%), and 25 were strongly (30%) biofilm positive. Seven isolates of S. aureus check for this species in other resources , 11 isolates of S.epidermidis check for this species in other resources , 2 isolates of S. warneri check for this species in other resources , 3 isolates of S.hominis check for this species in other resources , and 2 isolates of S.lugdunensis check for this species in other resources were identified as strong biofilm producers. Out of the 83 Staphylococcus isolates, 37 were cefuroxime, 18 ciprofloxacin, 11 vancomycin, 12 gatifloxacin, and 18 moxifloxacin resistant. In total, 37 strains were resistant to three or more antibiotics. There was a statistically significant relation between biofilm formation and multidrug resistance (against three or more antibiotics,p<0.001). In nondiabetic patients, 15(71%) isolates were non adherent or weakly adherent, and 2(10%) were strongly adherent biofilm positive.
In conclusion, bacterial conjunctival flora of patients with diabetes is likely to produce biofilm. Biofilm formation is associated with multidrug rsistance in patients with diabetes.

Diabetes; biofilm; Staphylococcus spp.; antibiotic resistance; ocular surface

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