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Medical Journal of The Islamic Republic of Iran
National Research Centre of Medical Sciences of I.R. IRAN
ISSN: 1016-1430
Vol. 20, No. 2, 2006, pp. 62-65 		Bioline Code: mr06015
Full paper language: English
Document type: Research Article
Document available free of charge

Medical Journal of The Islamic Republic of Iran, Vol. 20, No. 2, 2006, pp. 62-65

 en DETECTION OF CRYPTOCOCCUS NEOFORMANS check for this species in other resources BY SEMI-NESTED PCR IN CEREBROSPINAL FLUID
JAHROMI, SHAHINDOKHT BASSIRI; ABACHI, MANSOOR & KHAKSAR, ALI ASGHAR

Abstract

Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years. Cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (CNS) in immunocompetent as well as immunocompromised patients. We report the development of a semi-nested-PCR-based assay for the detection of C. neoformans in less than 100 yeast cells per ml of cerebrospinal fluid (CSF).

Keywords
Cryptococcus neoformans, PCR, Semi-nested PCR, CSF.

 
© Copyright 2006 -Medical Journal of the Islamic Republic of Iran
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