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African Journal of Food, Agriculture, Nutrition and Development
Rural Outreach Program
ISSN: 1684-5358
EISSN: 1684-5374
Vol. 9, No. 1, 2009, pp. 608-621
Bioline Code: nd09010
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Food, Agriculture, Nutrition and Development, Vol. 9, No. 1, 2009, pp. 608-621

 en Fatty Acid Composition and Amino Acid Profile of Two Freshwater Species, African Catfish ( Clarias gariepinus check for this species in other resources ) And Tilapia ( Tilapia zillii check for this species in other resources )
Osibona, A.O.; Kusemiju, K. & Akande, G.R.

Abstract

The proximate, fatty and amino acids composition of two commercially important freshwater fish species Clarias gariepinus check for this species in other resources and Tilapia zillii check for this species in other resources purchased from local fishermen in two landing sites in Lagos State, Nigeria were determined. Live specimens of C.gariepinus were purchased while samples of T. zilliiwere stored in an ice chest at 4°C and conveyed to the laboratory for analyses. The chemical composition of the samples was determined using AOAC methods. The protein content of C. gariepinus and T. zillii was 18.8% and 19.0% (% wet weight), respectively. The total lipid content was high for C. gariepinus (9.3%) but low for T. zillii (1.1) %. Lipid composition varied in the two species but differences were not statistically significant (P>0.05). The ash was 1.2% for both species. The moisture content was higher in T. zillii (80.4%) than in C. gariepinus (74.3%). The percentage composition of individual fatty acids as a ratio of total muscle lipids of C. gariepinus ranged from 0.1% to 26.0% and that of T. zillii from 0.1% to 32.2%. Among them, those occurring in the highest proportions were myristic acid (C14:0, 4.2-5.2%), palmitic acid (C16: 0, 22.0-32.2%), palmitoleic acid (C16:1, 3.6-13.2%), heptadecanoic acid (C17:0, 0.7-3.0%), stearic acid (C18:0, 8.1-9.5%), linoleic acid (C18:2, 1.4-12.3%) and oleic acid (C18:1). Amino acids from sample protein in C. gariepinus ranged from 0.30% to 17.81% and that of T. zilliifrom 0.27% to 18.16%. The most abundant amino acids in the two fish species were glutamic acid, aspartic acid, leucine and lysine ranging from 9.49% to 18.16%. The ratio of ω3/ω6 fatty acids was less than 1 in C. gariepinus while in T. zillii it was 2.70. The two freshwater fish species contain essential fatty acids particularly eicosapentaenoic acids and docosahexaenoic acids and essential amino acids for promoting good health, prevention and healing of diseases in humans. The two freshwater fish were therefore found to be important sources of nutrients.

Keywords
Proximate, Freshwater, fish, fattyacids, aminoacids.

 
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