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Neurology India
Medknow Publications on behalf of the Neurological Society of India
ISSN: 0028-3886
EISSN: 0028-3886
Vol. 52, No. 3, 2004, pp. 359-362
Bioline Code: ni04116
Full paper language: English
Document type: Research Article
Document available free of charge

Neurology India, Vol. 52, No. 3, 2004, pp. 359-362

 en Demonstration of IgG antibodies to 30 Kd protein antigen in CSF for diagnosis of tuberculous meningitis by antibody-capturing ELISA
Kashyap RS, Kainthla RP, Satpute RM, Chandak NH, Purohit HJ, Taori GM, Daginawala HF


Objective: A simple and rapid immunological assay method has been developed to demonstrate the presence of IgG antibodies to 30Kd protein antigen (30Kdpa) and culture filtrate protein (CFP) in the CSF of patients with Tuberculous meningitis (TBM).
Method: Antibody capturing Enzyme Linked Immunosorbent Assay (ELISA) was standardized with CFP antigen of MTB. The IgG antibodies were assayed in CSF sample from TBM and non-TBM patients against 30 Kdpa.
Results: The sensitivity and specificity of IgG antibodies for the diagnosis of suspected patients of TBM using 30 Kdpa was 80% and 91% respectively and the corresponding figures for CFP were 85% and 94% respectively. The sensitivity and specificity in two confirmed cases of TBM was 100%.
Conclusion: The presence of this 30Kdpa in the CSF of suspected cases of TBM consistently would indicate that the selected protein band carries the candidate protein marker antigen, which is specific to M. tuberculosis and could be considered as a diagnostic marker for TBM.

Tuberculous meningitis, mycobacterium tuberculosis, tubercular culture filtrate protein, 30 Kd protein antigen, cerebrospinal fluid, ELISA

© Copyright 2004 Neurology India.
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