Effect of naloxone on aluminum-induced learning and memory impairment in rats|
Shi-lei Sun, Guang-yu MA, Bachelor LIHua, Bachelor ZhuYun, Hong-mei Dong, Xiao-hu XU
Uptake of aluminum may disturb the learning and memory of humans or animals. Naloxone (NAL) has been shown to exert beneficial effects on memory deficits.
We investigated the effects of naloxone on aluminum-induced learning and memory impairment in rats.
SETTINGS AND DESIGN:
Aluminum-induced learning and memory impairment model was established by gavage of Aluminum chloride (600 mg/kg) for 3 months. Rats were divided into three groups viz. naloxone-treated rats (NAL 0.8 mg/kg, i.p. daily for 7 days), non-treated model rats and normal controls.
MATERIALS AND METHODS:
The Morris water maze test was performed to study spatial learning and memory. Long-term potentiation (LTP) of the Schaffer collateral-CA1 synapse was recorded. Aluminum and zinc contents in the hippocampus were assayed with atomic absorption spectrophotometry.
Parameters of the hidden and visible platform trials and data of LTP were analyzed using two-way repeated measures ANOVA.
In the hidden platform trials, escape latencies of the NAL rats were significantly shorter than that of the non-treated rats (P=0.000, 95% confidential interval low bound 14.31, upper bound 22.68). In probe trails, the number of entries in the target area of the NAL rats (6.75±1.28 times/min) was more than that of non-treated model rats (4.56±2.16 times/min, P=0.004, 95% confidence interval low bound -3.65, upper bound -0.788). The magnitudes of LTP recorded in the CA1 pyramidal neurons of the NAL-treated rats were significantly augmented when compared to the non-treated model rats (P=0.005, 95% confidence interval low bound 0.16, upper bound 0.84).
NAL could facilitate spatial learning and memory and enhance LTP in the CA1 region of the hippocampus in aluminum-induced learning and memory impairment in rats.
Naloxone, Aluminum, hippocampus, learning and memory, long-term potentiation, Morris water maze