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Neurology India
Medknow Publications on behalf of the Neurological Society of India
ISSN: 0028-3886
EISSN: 0028-3886
Vol. 58, No. 5, 2010, pp. 727-731
Bioline Code: ni10198
Full paper language: English
Document type: Research Article
Document available free of charge

Neurology India, Vol. 58, No. 5, 2010, pp. 727-731

 en Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis
Sharma, Kusum; Sharma, Aman; Singh, Malkit; Ray, Pallab; Dandora, Ritika; Sharma, Shiv K.; Modi, Manish; Prabhakar, Sudesh & Sharma, Meera


Background: Rapid and specific diagnosis of tubercular meningitis (TBM) is of utmost importance.
Aim: To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the diagnosis of tuberculous meningitis.
Materials and Methods: PCR using protein b primers was performed in ten patients with confirmed TBM (culture positive), 60 patients with clinically suspected TBM and 40 patients with no TBM (control group).
Results: Protein b PCR had a sensitivity of 90% and a specificity of 100% in patients with confirmed TBM. In 60 clinically diagnosed TBM patients, protein b PCR was positive in 49 (81.7%) patients. The overall sensitivity of microscopy, culture and PCR using protein b primers was 1.4%, 14.3%, and 82.8% and specificity was 100%, 100%, and 100% respectively.
Conclusion: Protein b PCR is valuable in rapid diagnosis of TBM.

Diagnosis, PCR, tubercular meningitis

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