About Bioline  All Journals  Testimonials  Membership  News

Neurology India
Medknow Publications on behalf of the Neurological Society of India
ISSN: 0028-3886
EISSN: 0028-3886
Vol. 59, No. 3, 2011, pp. 333-338
Bioline Code: ni11105
Full paper language: English
Document type: Research Article
Document available free of charge

Neurology India, Vol. 59, No. 3, 2011, pp. 333-338

 en Differentiation between the motor and sensory fascicles of the peripheral nerves from adult rats using annexin V-CdTe-conjugated polymer
Meng, Xianyu; Lu, Laijin; Wang, Hu & Liu, Bin


Background : Until now, no method has been available to rapidly differentiate between the motor and sensory nerve fascicles introperatively.
Aim : To establish a method to rapidly differentiate between the sensory and motor fascicles in the peripheral nerves.
Material and Methods : Annexin V-CdTe-conjugated polymer was used to stain the sciatic and sural nerve fascicles of adult male Wistar rats for 10, 15, 20, and 30 min.
Results : Under a light microscope, the sural nerves and the sensory fascicles of the sciatic were visualized as bright red fluorescence with Annexin V-CdTe-conjugated polymer staining. In contrast, no fluorescence on the motor fascicles of the sciatic nerve could be visualized. Fluorescence intensity was not strong enough to show the nerve fascicles with 10 min of staining; however, the intensity was clearly visible after 15 min of staining. No significant difference in the intensity of staining was observed among samples stained for 15, 20, and 30 min.
Conclusions : Our study shows that Annexin V-CdTe-conjugated polymer can differentiate the motor and sensory nerve fascicles of the peripheral nerve rapidly and precisely in vitro. This technique represents a new method for the identification of peripheral nerve fascicles.

Annexin V, CdTe, differentiate, peripheral nerve fascicles, quantum dots

© Copyright 2011 Neurology India.
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil