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Nigerian Journal of Physiological Sciences
Physiological Society of Nigeria
ISSN: 0794-859X
Vol. 22, No. 1-2, 2007, pp. 129-134
Bioline Code: np07022
Full paper language: English
Document type: Research Article
Document available free of charge

Nigerian Journal of Physiological Sciences, Vol. 22, No. 1-2, 2007, pp. 129-134

 en MORINDA LUCIDA check for this species in other resources REDUCES CONTRACTILITY OF ISOLATED UTERINE SMOOTH MUSCLE OF PREGNANT AND NON-PREGNANT MICE
ELIAS, S. O.; LADIPO, C. O.; ODUWOLE, B. P.; EMEKA,P. M.; OJOBOR, P. D. & SOFOLA, O. A.

Abstract

The present work investigated the effect of MORINDA LUCIDA check for this species in other resources (M. lucida) extract on isolated uterine smooth muscle of pregnant and non-pregnant mice. Pregnant and non-pregnant mice were pretreated with oral stilboesterol (0.1mg/kg body weight) and killed by cervical dislocation. Thin strips of the uterus were cut and mounted in a 20-ml organ bath containing De Jalon solution bubbled with 95%O2-5% CO2 gas mixture. The strips were connected to a force transducer coupled to a Grass 7D Polygraph for the recording of isometric tension. Effects of graded concentrations of oxytocin (OXY; 10-5–10-2 mol/L), acetylcholine (ACh; 10-9-10-5 mol/L) and M. lucida extract (0.015–1.5mg/ml) were recorded. Fresh uterine strips were then incubated with M. lucida extract for 5mins and cumulative response to OXY was repeated. Another set of fresh strips was incubated in L-NAME for 15mins and the cumulative responses to M.lucida extract were repeated. OXY resulted in increased contractile responses in both pregnant and non-pregnant uterine muscles. M. lucida resulted in relaxation of the uterine smooth muscle in both pregnant and non-pregnant mice at all doses. However, at 1.500mg/ml, M. lucida completely blocked spontaneous uterine contractions. Following incubation with L-NAME, M. lucida extract led to a slightly greater relaxation of the uterine strips. In conclusion, M. lucida reduced contractility of uterine smooth muscle in both pregnant and non-pregnant mice as well as blocking contractile responses to OXY and Ach in uterine smooth muscle of pregnant and non-pregnant mice. There was no significant alteration of M. lucida activity by L-NAME suggesting that the action of the compound on uterine muscle is not associated with impaired nitric oxide synthase.

Keywords
Morinda lucida, uterine contraction, nitric oxide, mice

 
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