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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 1, 2000, pp. 95-96
Bioline Code: oc00015
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 1, 2000, pp. 95-96

 en Short Communication - Cellulose Acetate as Solid Phase in ELISA for Plague
AD Barbosa; FSM de Barros; EQ Callou; AMP Almeida; AM Araujo; WM Azevedo & LB Carvalho Jr

Abstract

Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 g of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.

Keywords
cellulose acetate - Yersinia pestis - ELISA

 
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