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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 1, 2000, pp. 97-102
Bioline Code: oc00016
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 1, 2000, pp. 97-102

 en Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi
Rosalia Agusti; Alicia S Couto; Maria Júlia M Alves; Walter Colli & Rosa M de Lederkremer

Abstract

Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C{SUB}16:1{/SUB} and C{SUB}18:1{/SUB} when compared to the total fatty acid pool isolated from the parasites.

Keywords
[14C]-ethanolamine and [3H]-palmitic acid incorporation - phospholipids - desaturated fatty acids

 
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