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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 221-223
Bioline Code: oc00037
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 221-223

 en Short Communication: A Simple Method for Human Peripheral Blood Monocyte Isolation
Marcos C de Almeida; Alan C Silva; Aldina Barral & Manoel Barral Netto


We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes. {P}Key words:

monocytes - percoll - gradient-isolation

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