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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 225-226
Bioline Code: oc00038
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 225-226

 en Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
AL Cândido; ED Bontempo & M Resende

Abstract

Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.

Keywords
bovine herpesvirus-1 - cosolvents - polymerase chain reaction

 
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