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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 231-235
Bioline Code: oc00040
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 231-235

 en An in Vitro System from Plasmodium falciparum Active in Endogenous mRNA Translation
Ana Ferreras; Ledia Triana; Heriberto Correia; Erlinda Sánchez & Flor Herrera

Abstract

An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.

Keywords
Plasmodium falciparum - protein synthesis - cell free translation - parasite lysate

 
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