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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 265-270
Bioline Code: oc00046
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 265-270

 en Ultrastructural Study of the TG180 Murine Sarcoma Cell Invasion by Toxoplasma gondii: Comparison between in Vivo and in Vitro Cell Cultures
Hugo Marcelo Ribeiro Barbosa; Marcos Silva; Eloisa Amália Vieira Ferro & José Roberto Mineo

Abstract

Infection of non-adherent TG180 murine sarcoma cells with Toxoplasma gondii was compared, at the ultrastructural level, in both in vivo and in vitro conditions. Suspensions of 3.0 x 106 TG180 cells infected in vitro with 1.0 x 106 parasites of the RH strain were harvested between the first and 6th day post-infection and processed for transmission electron microscopy. In vivo infection was made by intraperitoneal inoculation in mice of 1.0 x 106 TG180 cells, that were co-inoculated with a parasite suspension at the same cell concentration. Cells were harvested 10, 20, 30 min and 24, 48 h post-inoculation and processed for transmission electron microscopy at the same conditions of the in vitro culture. It was observed TG180 murine sarcoma cells with intense and equivalent intracellular parasitism in both conditions. Host cells with parasitophorous vacuoles containing up to 16 parasites, as well as parasites undergoing mitoses or presenting a bradyzoite-like morphology, were frequently seen in both culture methods.

Keywords
Toxoplasma gondii - TG180 murine sarcoma cells - in vitro culture - transmission electron microscopy

 
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