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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 3, 2000, pp. 323-327
Bioline Code: oc00054
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 3, 2000, pp. 323-327

 en Direct Methods for Detecting Picorna-like Virus from Dead and Alive Triatomine Insects
GS Rozas Dennis; JL La Torre; OA Muscio & DMA Gurin

Abstract

In this work we report four different destructive and non-destructive methods for detecting picorna-like virus particles in triatomines. The methods are based on direct observation under transmission electron microscope and they consist of four ways to prepare samples of presumable infected material. The samples are prepared processing dead or alive insect parts, or even dry or fresh insect feces. The methods can be used as analytical or preparative techniques, for quantifying virus infection and checking virus integrity as well. In this work the four methods are applied in order to detect Triatoma virus (TrV) particles in T. infestans colonies.

Keywords
picorna-like virus, Triatoma virus, Triatoma infestans, transmission electron microscope methods

 
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