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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 3, 2000, pp. 401-402
Bioline Code: oc00065
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 3, 2000, pp. 401-402

 en Detection of Intracytoplasmic Cytokines by Flow Cytometry
Marta A Santiago; Paula M De Luca; lvaro L Bertho; Rilza BG Azeredo-Coutinho & Srgio G Coutinho


Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.

intracellular cytokines, flow cytometry, leishmaniasis

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