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Detection of Intracytoplasmic Cytokines by Flow Cytometry Marta A Santiago; Paula M De Luca; lvaro L Bertho; Rilza BG Azeredo-Coutinho & Srgio G Coutinho Abstract Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models. Keywords intracellular cytokines, flow cytometry, leishmaniasis

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