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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 6, 2000, pp. 801-806
Bioline Code: oc00128
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 6, 2000, pp. 801-806

 en Analysis of Parity Between Protein-based Electrophoretic Methods for the Characterization of Oral Candida Species
EAR Rosa; RT Rosa; CV Pereira; MFG Boriollo & JF Höfling

Abstract

Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.

Keywords
polyacrylamide gel electrophoresis, multilocus enzyme electrophoresis, Candida,numerical analysis

 
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