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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 6, 2000, pp. 863-866
Bioline Code: oc00140
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 6, 2000, pp. 863-866

 en A Simplified Method for Sample Collection and DNA Isolation for Polymerase Chain Reaction Detection of Trypanosoma rangeli and Trypanosoma cruzi in Triatomine Vectors
Evandro MM Machado; Nelson J Alvarenga; Alvaro J Romanha & Edmundo C Grisard

Abstract

Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America, sharing several reservoirs and triatomine vectors, we herein describe a simple method to collect triatomine feces and hemolymph in filter paper for further detection and specific characterization of these two trypanosomes. Experimentally infected triatomines feces and hemolymph were collected in filter paper and specific detection of T. rangeli or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA collection method allows sample collection in the field and further specific trypanosome detection and characterization in the laboratory.

Keywords
Trypanosoma cruzi, Trypanosoma rangeli, polymerase chain reaction, triatomines, Dipetalogaster maximus

 
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