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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 96, No. 2, 2001, pp. 233-235
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Bioline Code: oc01033
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 2, 2001, pp. 233-235
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Antigen Incorporation on Cryptosporidium parvum Oocyst Walls
Emilio Entrala; Younes Sbihi; Manuel Sánchez-Moreno & Carmen Mascaró
Abstract
Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (Merifluor, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism.
Keywords
Cryptosporidium, oocyst wall, antigen
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© Copyright 2001 Fundacao Oswaldo Cruz Fiocruz Alternative site location: http://memorias.ioc.fiocruz.br
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