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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 2, 2001, pp. 241-245
Bioline Code: oc01035
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 2, 2001, pp. 241-245

 en Enzyme-linked Immunosorbent Assay for Detection and Quaffigntification of Cryptococcus neoformans Antigen
Ma. Teresa Illnait; Juan C Vilaseca; Carlos M Fernández & Gerardo F Martínez

Abstract

An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.

Keywords
cryptococcal antigen, antigen quantification, antigen detection, enzyme-linked immunosorbent assay-ELISA

 
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