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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 3, 2001, pp. 381-383
Bioline Code: oc01057
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 3, 2001, pp. 381-383

 en Resistance of Biomphalaria occidentalis from Varzea das Flores Dam, Minas Gerais, to Schistosoma mansoni Infection Detected by Low Stringency Polymerase Chain Reaction
Cecilia Pereira de Souza & Liana K Jannotti Passos

Abstract

Biomphalaria occidentalis Paraense, 1981 from Varzea das Flores dam, MG, Brazil, was exposed to infection with Schistosoma mansoni. Individual infection was performed with 140 B. occidentalis and 100 B. glabrata snails using LE and SJ strains. Two groups of B. occidentalis were killed after seven day-miracidia exposure to detect S. mansoni DNA, through the low stringency polymerase chain reaction (LS-PCR), and were negative. The infection rates were 69.2% (LE strain) and 96.7% (SJ strain) for B. glabrata and 0% for B. occidentalis. LS-PCR enabled early resistance diagnosis.

Keywords
Schistosoma mansoni, Biomphalaria occidentalis, resistance, Minas Gerais

 
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