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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 3, 2001, pp. 435-436
Bioline Code: oc01066
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 3, 2001, pp. 435-436

 en Genetic Markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) Obtained by the Double Stringency Polymerase Chain Reaction Technique
Roseli Tuan & Paula Cristina Bortolato

Abstract

Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.

Keywords
Biomphalaria, DNA polymorphism, PCR

 
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