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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 4, 2001, pp. 535-544
Bioline Code: oc01082
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 4, 2001, pp. 535-544

 en Genetic Variability in Brazilian Populations of Biomphalaria straminea Complex Detected by Simple Sequence Repeat Anchored Polymerase Chain Reaction Amplification
Roberta L Caldeira; Teofânia HDA Vidigal; Andrew JG Simpson & Omar S Carvalho

Abstract

Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to morphological similarities, has been grouped with B. intermedia and B. kuhniana in a complex named B. straminea. In the current work, we have standardized the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) technique, using the primers (CA)8RY and K7, to study the genetic variability of these species. The similarity level was calculated using the Dice coefficient and genetic distance using the Nei and Li coefficient. The trees were obtained by the UPGMA and neighbor-joining methods. We have observed that the most related individuals belong to the same species and locality and that individuals from different localities, but of the same species, present clear heterogeneity. The trees generated using both methods showed similar topologies. The SSR-PCR technique was shown to be very efficient in intrapopulational and intraspecific studies of the B. straminea complex snails.

Keywords
snails, Planorbidae, Biomphalaria straminea, Biomphalaria kuhniana, Biomphalaria intermedia, SSR anchored PCR amplification, genetic variability

 
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