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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 4, 2001, pp. 583-586
Bioline Code: oc01088
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 4, 2001, pp. 583-586

 en A Comparative Study of Preservation and Storage of Haemophilus influenzae
Olga C Aulet de Saab; Marta C de Castillo; Aida P de Ruiz Holgado & Olga M de Nader

Abstract

The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20oC and -70oC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20°C and -70°C from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20°C. Initial concentration did not influence survival after storage at -70°C. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.

Keywords
Haemophilus influenzae, storage, culture media

 
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