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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 5, 2001, pp. 661-665
Bioline Code: oc01102
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 5, 2001, pp. 661-665

 en Identification of Biomphalaria havanensis and Biomphalaria obstructa Populations from Cuba Using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of the Ribosomal RNA Intergenic Spacer
Teofânia HDA Vidigal; Roberta L Caldeira; Andrew JG Simpson & Omar S Carvalho


In Cuba, several Biomphalaria species have been reported such as B. orbignyi, B. schrammi, B. helophila, B. havanensis and B. peregrina; only the latter three are considered as potential hosts of Schistosoma mansoni. The specific identification of Biomphalaria species is based on anatomical and morphological characters of genital organs and shells. The correct identification of these snails is complicated by the high variation in these characters, similarity among species and in some cases by the small size of the snails. In this paper, we reported the classical morphological identification, the use of PCR and RFLP analysis of the internal transcribed spacer region of the ribosomal RNA genes for molecular identification of seven snail populations from different localities in Cuba. Using morphological and molecular analysis, we showed that among the studied Cuban Biomphalaria populations only B. havanensis and B. obstructa species were found.

Biomphalaria havanensis, Biomphalaria obstructa, polymerase chain reaction, internal transcribed spacer, ribosomal DNA, snails, Cuba

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