search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 5, 2001, pp. 693-695
Bioline Code: oc01109
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 5, 2001, pp. 693-695

 en SHORT COMMUNICATION - Comparison between Precipitin and ELISA Tests in the Bloodmeal Detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) Mosquitoes Experimentally Fed on Feline, Canine and Human Hosts
Luciana AM Gomes; Rosemere Duarte; Dinair C Lima; Beatriz Sanson Diniz; Maria Lucia Serrão & Norma Labarthe

Abstract

The identification of arthropod bloodmeals is important in many epidemiological studies, as, the understanding of the life cycle of vectors and the patogens they transmit, as well as helping to define arthropods' control strategies. The precipitin test has been used for decades, but ELISA is slowly becoming more popular. To compare the two tests for sensitivity, specificity and accuracy to detect small insect bloodmeals, Aedes aegypti or Ae. fluviatilis mosquitoes were fed either on feline, canine or human hosts. Mosquitoes were frozen at 6, 12, 24, 48 or 72 h after feeding. Precipitin test showed better specificity and accuracy and ELISA test showed higher sensitivity. Better results with both tests were achieved when mosquitoes were frozen within 48 h from feeding.

Keywords
bloodmeal identification, mosquito, ELISA, canine blood, feline blood, human blood

 
© Copyright 2001 Mem Inst Oswaldo Cruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil