|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 96, No. 6, 2001, pp. 815-821
|
Bioline Code: oc01131
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 6, 2001, pp. 815-821
en |
An Improved Method for Concentrating Rotavirus from Water Samples
Leera Kittigul; Porntip Khamoun; Dusit Sujirarat; Fuangfa Utrarachkij; Kitja Chitpirom; Nattasai Chaichantanakit & Kanda Vathanophas
Abstract
A modified adsorption-elution method for the concentration of seeded rotavirus from water samples was used
to determine various factors which affected the virus recovery. An enzyme-linked immunosorbent assay was
used to detect the rotavirus antigen after concentration. Of the various eluents compared, 0.05M glycine, pH 11.5 gave the highest rotavirus antigen recovery using negatively charged membrane filtration whereas
2.9% tryptose phosphate broth containing 6% glycine; pH 9.0 was found to give the greatest elution
efficiency when a positively charged membrane was used. Reconcentration of water samples by a speedVac
concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through
both negatively and positively charged filters (p-value {0.001). In addition, speedVac concentration using
negatively charged filtration resulted in greater rotavirus recovery than that using positively charged filtration (p-value = 0.004). Thirty eight environmental water samples were collected from river, domestic sewage, canals receiving raw sewage drains, and tap water collected in containers for domestic use, all from congested areas of Bangkok. In addition, several samples of commercial drinking water were analyzed. All samples were concentrated and examined for rotavirus antigen. Coliforms and fecal coliforms (0-}1,800
MPN/100 ml) were observed but rotavirus was not detected in any sample. This study suggests that the
speedVac reconcentration method gives the most efficient rotavirus recovery from water samples.
Keywords
rotavirus, speedVac reconcentration, water samples
|
|
© Copyright 2001 Mem Inst Oswaldo Cruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|