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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 6, 2001, pp. 835-838
Bioline Code: oc01135
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 6, 2001, pp. 835-838

 en SHORT COMMUNICATION - Identification of a Differentially Expressed mRNA in Axenic Leishmania panamensis Amastigotes
José Arturo Gutiérrez; Fabiola Puentes; Alberto Moreno; Manuel Elkin Patarroyo & Luis Angel Murillo

Abstract

Differential display technique was applied in order to identify transcripts which are present in axenic amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and the sequence reveals an open reading frame of 364 amino acids (aprox. 40 kDa). The deduced protein is homologous to the serine/threonine protein kinases and specially to the mitogen activates protein kinases from eukaryotic species. Southern blot analysis suggest that this transcript, named lpmkh, is present in the genome of the parasite as a single copy gene. These results could imply that lpmkh could be involved in the differentiation process or the preservation of amastigotes in axenic conditions.

Keywords
differential display, axenic amastigotes, Leishmania panamensis, mitogen activated protein kinase

 
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