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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 7, 2001, pp. 901-902
Bioline Code: oc01177
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 7, 2001, pp. 901-902

 en SHORT COMMUNICATION - Isolation of Leishmania infantum check for this species in other resources , Zymodeme MON-1 from Canine and Human Visceral Leishmaniasis on Margarita Island, Venezuela
Olga Zerpa; Francine Pratlong; Marian Ulrich & Jacinto Convit

Abstract

An increase in the incidence of human visceral leishmaniasis (HVL) has been detected in recent years on Margarita Island, located off the NE coast of Venezuela. Recent studies have revealed reactivity to rK39 antigen ( Leishmania chagasi check for this species in other resources ) in 20% of 541 sera from domestic dogs in endemic communities; PCR reactions were positive using primers for the L. donovani complex. Here we report that isolates from human and canine infection, identified by isoenzyme analysis, correspond to L. infantum, zymodeme MON-1. This appears to be the first isolation and identification of an isolate from HVL on Margarita Island and demonstrates the presence of this zymodeme in the canine population.

Keywords
visceral leishmaniasis, Leishmania infantum MON-1, Venezuela

 
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