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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 7, 2001, pp. 973-978
Bioline Code: oc01189
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 7, 2001, pp. 973-978

 en Mycobacterium bovis check for this species in other resources BCG but not Mycobacterium leprae check for this species in other resources Induces TNF-α Secretion in Human Monocytic THP-1 Cells
Martha M Oliveira; Rosane Charlab & Maria Cristina V Pessolani

Abstract

In this study, we compared the level of TNF-α secretion induced in monocytic THP-1 cells after phagocytosis of Mycobacterium leprae check for this species in other resources , the causative agent of leprosy, and M. bovis BCG, an attenuated strain used as a vaccine against leprosy and tuberculosis. The presence of M. leprae and BCG was observed in more than 80% of the cells after 24 h of exposure. However, BCG but not M. leprae was able to induce TNF-α secretion in these cells. Moreover, THP-1 cells treated simultaneously with BCG and M. leprae secreted lower levels of TNF-α compared to cells incubated with BCG alone. M. leprae was able, however, to induce TNF-α secretion both in blood-derived monocytes as well as in THP-1 cells pretreated with phorbol myristate acetate. The inclusion of streptomycin in our cultures, together with the fact that the use of both gamma-irradiated M. leprae and heat-killed BCG gave similar results, indicate that the differences observed were not due to differences in viability but in intrinsic properties between M. leprae and BCG. These data suggest that the capacity of M. leprae to induce TNF-α is dependent on the stage of cell maturation and emphasize the potential of this model to explore differences in the effects triggered by vaccine strain versus pathogenic species of mycobacteria on the host cell physiology and metabolism.

Keywords
Mycobacterium leprae - BCG - THP-1 - TNF-α

 
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