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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 96, No. 8, 2001, pp. 1043-1048
Bioline Code: oc01202
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 8, 2001, pp. 1043-1048

 en Differentiation and Genetic Analysis of Rhodnius prolixus check for this species in other resources and Rhodnius colombiensis check for this species in other resources by rDNA and RAPD Amplification
Carlos Jaramillo; María Fernanda Montaña; Lyda Raquel Castro; Gustavo A Vallejo & Felipe Guhl

Abstract

Domiciliated Rhodnius prolixus check for this species in other resources and sylvatic R. colombiensis check for this species in other resources were analyzed in order to confirm their genetic divergence and verify the risk that the latter represents in the domiciliation process, and to provide tools for identifying the sources of possible reinfestation by triatomines in human dwellings allowing control programs to be undertaken. Comparison of random amplified polymorphic DNA amplification patterns and cluster analysis suggests reproductive discontinuity between the two species. The calculated statistical F value of 0.24 and effective migration rate of 0.6 individuals per generation are insufficient to maintain genetic homogeneity between them and confirm the absence of present genetic flow. R. colombiensis presents higher intrapopulation variability. Polymerase chain reaction of ribosomal DNA supports these findings. The low genetic flow between the two species implies that R. colombiensis do not represent an epidemiological risk for the domiciliary transmission of Trypanosoma cruzi in the Tolima Department. The lower variability of the domiciliated R. prolixus could result in greater susceptibility to the use of pesticides in control programs.

Keywords
Rhodnius prolixus, Rhodnius colombiensis, domiciliated and sylvatic triatomines, molecular markers, random amplified polymorphic DNA, ribosomal genes, control programs

 
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