Lutzomyia check for this species in other resources spp. - phlebotomines - Leishmania braziliensis check for this species in other resources - kDNA PCR - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 2, 2002, pp. 185-188
Bioline Code: oc02033
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 2, 2002, pp. 185-188

 en Frequency of Infection of Lutzomyia check for this species in other resources Phlebotomines with Leishmania braziliensis check for this species in other resources in a Brazilian Endemic Area as Assessed by Pinpoint Capture and Polymerase Chain Reaction
José Carlos Miranda; Eliana Reis; Albert Schriefer; Marilda Gonçalves; Mitermayer Galvão Reis; Lucas Carvalho; Octavio Fernandes*; Manoel Barral-Netto & Aldina Barral

Abstract

Leishmania check for this species in other resources infected of Lutzomyia check for this species in other resources spp. are rare in endemic areas. We tested the hypothesis that there is clustering of infected vectors by combining pinpoint capture with sensitive L. braziliensis check for this species in other resources kDNA minicircle specific PCR/dot blot in an endemic area in the State of Bahia. Thirty out of 335 samples (10 to 20 sand flies/sample; total of 4,027 female sand flies) were positive by PCR analysis and dot blot leading to a underestimated overall rate of 0.4% positive phlebotomines. However, 83.3% of the positive samples were contributed by a single sector out of four sectors of the whole studied area. This resulted in a rate of 1.5% Leishmania positive phlebotomines for this sector, far above rates of other sectors. Incidence of American cutaneous leishmaniasis cases for this sector was about twice that for other sectors. Our results show that there is a non-homogeneous distribution of Leishmania-infected vectors. Such a clustering may have implications in control strategies against leishmaniasis, and reinforces the necessity of understanding the ecological and geographical factors involved in leishmanial transmission.

Keywords
Lutzomyia check for this species in other resources spp. - phlebotomines - Leishmania braziliensis check for this species in other resources - kDNA PCR - Brazil

 
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