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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 4, 2002, pp. 517-522
Bioline Code: oc02098
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 4, 2002, pp. 517-522

 en Anionic Sites, Fucose Residues and Class I Human Leukocyte Antigen Fate During Interaction of Toxoplasma gondii check for this species in other resources with Endothelial Cells
Ana Carolina Stumbo; Helene Santos Barbosa; Técia Maria Ulisses de Carvalho; Luís Cristóvão Porto & Laís de Carvalho

Abstract

Toxoplasma gondii check for this species in other resources invades and proliferates in human umbilical vein endothelial cells where it resides in a parasitophorous vacuole. In order to analyze which components of the endothelial cell plasma membrane are internalized and become part of the parasitophorous vacuole membrane, the culture of endothelial cells was labeled with cationized ferritin or UEA I lectin or anti Class I human leukocytte antigen (HLA) before or after infection with T. gondiii . The results showed no cationized ferritin and UEA I lectin in any parasitophorous vacuole membrane, however, the Class I HLA molecule labeling was observed in some endocytic vacuoles containing parasite until 1 h of interaction with T. gondiii . After 24 h parasite-host cell interaction, the labeling was absent on the vacuolar membrane, but presents only in small vesicles near parasitophorous vacuole. These results suggest the anionic site and fucose residues are excluded at the time of parasitophorous vacuole formation while Class I HLA molecules are present only on a minority of Toxoplasma-containig vacuoles.

Keywords
Toxoplasma gondii check for this species in other resources - endothelial cells - ferritin - Ulex europaeusi check for this species in other resources agglutinin-1 - histocompatibility antigens Class I

 
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