Euphorbia check for this species in other resources ceae - ethnobotany - medicinal plants - antiviral - herpes simplex virus - Colombia"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 4, 2002, pp. 541-546

Bioline Code: oc02103
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 4, 2002, pp. 541-546

 en Cytotoxic and Antiviral Activities of Colombian Medicinal Plant Extracts of the Euphorbia check for this species in other resources genus
LA Betancur-Galvis; GE Morales; JE Forero & J Roldan

Abstract

Forty-seven plant extracts of 10 species of the genus Euphorbia check for this species in other resources ( Euphorbia ceae) used by Colombian traditional healers for the treatment of ulcers, cancers, tumors, warts, and other diseases, were tested in vitro for their potential antitumour (antiproliferative and cytotoxic) and antiherpetic activity. To evaluate the capacity of the extracts to inhibit the lytic activity of herpes simplex virus type 2 (HSV-2) and the reduction of viability of infected or uninfected cell cultures, the end-point titration technique (EPTT) and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay were used, respectively. The therapeutic index of the positive extracts for the antiviral activity was determined by calculating the ratio CC 50 (50% cytotoxic concentration) over IC 50 (50% inhibitory concentration of the viral effect). Five of the 47 extracts (11%) representing 3 out of 10 Euphorbia species (30%) exhibited antiherpetic action; the highest activity was found in the leaf/stem water-methanol extracts from E. cotinifolia check for this species in other resources and E. tirucalli check for this species in other resources . The therapeutic indexes of these two plant species were > 7.1; these extracts exhibited no cytotoxicity. Six extracts (13%) representing 4 plant species (40%) showed cytotoxic activity. The highest cytotoxicity was found in the dichloromethane extract obtained from E. cotinifolia leaves and the CC 50 values for the most susceptible cell lines, HEp-2 and CHO, were 35.1 and 18.1 mg/ml, respectively.

Keywords
Euphorbia check for this species in other resources ceae - ethnobotany - medicinal plants - antiviral - herpes simplex virus - Colombia

 
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