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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 5, 2002, pp. 695-701
Bioline Code: oc02134
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 5, 2002, pp. 695-701

 en Intergenic and External Transcribed Spacers of Ribosomal RNA Genes in Lizard-infecting Leishmania check for this species in other resources : Molecular Structure and Phylogenetic Relationship to Mammal-infecting Leishmania in the Subgenus Leishmania ( Leishmania )
Tereza C Orlando; Mary Anne T Rubio; Nancy R Sturm; David A Campbell & Lucile M Floeter-Winter


To establish the relationships of the lizard- and mammal-infecting Leishmania check for this species in other resources , we characterized the intergenic spacer region of ribosomal RNA genes from L. tarentolae and L. hoogstraali . The organization of these regions is similar to those of other eukaryotes. The intergenic spacer region was approximately 4 kb in L. tarentolae and 5.5 kb in L. hoogstraali . The size difference was due to a greater number of 63-bp repetitive elements in the latter species. This region also contained another element, repeated twice, that had an inverted octanucleotide with the potential to form a stem-loop structure that could be involved in transcription termination or processing events. The ribosomal RNA gene localization showed a distinct pattern with one chromosomal band (2.2 Mb) for L. tarentolae and two (1.5 and 1.3 Mb) for L. hoogstraali . The study also showed sequence differences in the external transcribed region that could be used to distinguish lizard Leishmania from the mammalian Leishmania . The intergenic spacer region structure features found among Leishmania species indicated that lizard and mammalian Leishmania are closely related and support the inclusion of lizard-infecting species into the subgenus Sauroleishmania proposed by Saf'janova in 1982.

lizard leishmania - RNA polymerase I - rRNA processing - repetitive DNA - rRNA spacer evolution

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