Cryptosporidium check for this species in other resources - Crotalus durissus terrificus check for this species in other resources - snakes - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 6, 2002, pp. 779-781
Bioline Code: oc02149
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 6, 2002, pp. 779-781

 en Occurrence of Cryptosporidium check for this species in other resources (Apicomplexa, Cryptosporidiidae) in Crotalus durissus terrificus check for this species in other resources (Serpentes, Viperidae) in Brazil
Andréa Satie Matsubara Karasawa; Reinaldo José da Silva; Luciene Maura Mascarini; Thomaz Henrique Barrella & Carlos Alberto de Magalhães Lopes

Abstract

The objective of the present study was to investigate the prevalence of Cryptosporidium check for this species in other resources (Apicomplexa, Cryptosporidiidae) in the snake Crotalus durissus terrificus check for this species in other resources (Serpentes, Viperidae). Fifty animals were evaluated for the presence of oocysts of Cryptosporidium sp. at the time of arrival and 30 and 60 days later. Intestinal washings with saline solution (1% body weight), fecal samples, and organ scrapings were collected during the study. Oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by a density gradient centrifugation technique. Smears were made with the sediment and submitted to modified acid-fast and auramine-rhodamine staining. Cryptosporidium -positive smears were used as controls for the experimental findings. The overall prevalence of Cryptosporidium sp. oocysts was 14%. Among the positive snakes, oocysts were detected only in the intestinal washing in two specimens, only in the feces in four specimens, and in both materials at least once in one specimen. The positive snakes were predominantly from Santa Maria da Serra city State of São Paulo (57.1%). We also observed that all of the examinations that presented positive results were obtained at least 27 days after the capture of the animals.

Keywords
Cryptosporidium check for this species in other resources - Crotalus durissus terrificus check for this species in other resources - snakes - Brazil

 
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