Aedes aegypti check for this species in other resources - Aedes albopictus check for this species in other resources - reverse transcription-polymerase chain reaction - virus isolation - Rio de Janeiro - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 6, 2002, pp. 799-800
Bioline Code: oc02154
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 6, 2002, pp. 799-800

 en SHORT COMMUNICATION - Dengue Virus Type 3 Isolation from Aedes aegypti check for this species in other resources in the Municipality of Nova Iguaçu, State of Rio de Janeiro
Ricardo Lourenço-de-Oliveira; Nildimar A Honório; Márcia G Castro; Hermann G Schatzmayr; Marize P Miagostovich; João CR Alves; Wellington C Silva; Paulo J Leite & Rita MR Nogueira

Abstract

In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti check for this species in other resources and Ae. albopictus check for this species in other resources mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of Ae. aegypti , despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that area. No viruses were detected in Ae. albopictus mosquitoes. This virological surveillance consists in a sentinel system alerting for dengue outbreaks.

Keywords
Aedes aegypti check for this species in other resources - Aedes albopictus check for this species in other resources - reverse transcription-polymerase chain reaction - virus isolation - Rio de Janeiro - Brazil

 
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