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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 6, 2002, pp. 857-864
Bioline Code: oc02164
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 6, 2002, pp. 857-864

 en Changes in Nuclear Phenotype Frequencies Following Sequential Cold Shocks in Triatoma infestans check for this species in other resources (Hemiptera, Reduviidae)
Silvana GP Campos; Vera Lúcia CC Rodrigues & Maria Luiza S Mello

Abstract

The nuclear phenotypes of Malpighian tubule cells in fifth instar nymphs of Triatoma infestans check for this species in other resources , one of the most important vectors of Chagas disease, were studied following sequential shocks at 0ºC, separated by intervals of 8 h and 24 h at 30ºC, under conditions of moderate fasting and full nourishment. The insects pertained to colonies reared in the laboratory and originated from domestic specimens collected in the Brazilian states of São Paulo (north) and Minas Gerais (south). Since nuclear phenotypes in this species are affected by single cold shocks, it was expected that these phenotypes could also be changed by sequential shocks. Nuclear phenotypes indicative of mechanisms of cell survival (nuclear fusion and heterochromatin decondensation) and cell death (apoptosis and necrosis) were observed concomitantly in all the conditions tested. Nuclear fusion and heterochromatin decondensation were not found relevant for the presumed acquisition of the cold-hardening response in T. infestans . The decreased frequency of apoptosis and necrosis following sequential cold shocks including under fasting conditions, indicated that tolerance to sequential cold shocks occurred in T. infestans of the mentioned origin.

Keywords
cold shocks - sequential shocks - nuclear phenotypes - Triatoma infestans check for this species in other resources

 
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