Trypanosoma cruzi check for this species in other resources - internal transcribed spacers - mini-exon gene - ribosomal DNA - Colombia"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. 6, 2002, pp. 877-880
Bioline Code: oc02168
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 6, 2002, pp. 877-880

 en SHORT COMMUNICATION - Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi check for this species in other resources Isolates Revealed by the Ribosomal DNA
Patricia Cuervo; Elisa Cupolillo; Iris Segura; Nancy Saravia & Octavio Fernandes

Abstract

American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi check for this species in other resources presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli . In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.

Keywords
Trypanosoma cruzi check for this species in other resources - internal transcribed spacers - mini-exon gene - ribosomal DNA - Colombia

 
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