Eighteen clinical isolates of
were obtained from women who attended health centers of the Goverment of
Madrid. A total of 1,848 vaginal specimens recovered during the gynaecological
examination were seeded in culture tubes containing liquid Diamond medium.
Pathogenicity to mice was determined after intraperitoneal inoculation of
mice by quantification of mortality and gross damage to abdominal organs.
As could be expected, a broad variability was obtained, being some of the
isolates more virulent than the reference strain. Regarding to metronidazole
susceptibility, none resistant isolate was found but different degrees of
susceptibility were determined.