|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 97, No. 6, 2002, pp. 909-913
|
Bioline Code: oc02175
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 97, No. 6, 2002, pp. 909-913
en |
Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
Denise Cristina Souza Matos; Rugimar Marcovistz; Pedro Hernan Cabello; Ricardo Amaral Georgini; Dirce Sakauchi & Luciana Leite da Silva
Abstract
Samples from 20 lots of diphtheria-tetanus (adult use dT)
vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were
used to standardize and validate the in vitro toxin binding inhibition (ToBI)
test for the immunogenicity test of the tetanus component. The levels of
tetanus antitoxin obtained by ToBI test were compared to those obtained
using the toxin neutralization (TN) test in mice routinely employed to perform
the quality control of the tetanus component in adsorbed vaccines. The results
ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test
and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These
results were significantly correlated. From this study, it is concluded
that the ToBI test is an alternative to the in vivo neutralization procedure
in the immunogenicity test of the tetanus component in adsorbed vaccines.
A substantial refinement and a reduction in use of animals can be achieved.
Keywords
diphtheria-tetanus vaccine - diphtheria-tetanus-pertussis vaccine - toxin binding inhibition test - tetanus - quality control - seroneutralization
|
|
© Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|