Bulinus check for this species in other resources - cytochrome oxidase - single nucleotide polymorphism - schistosomiasis - Schistosoma haematobium check for this species in other resources "/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 31-36
Bioline Code: oc02183
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 31-36

 en Identification of Snails within the Bulinus africanus check for this species in other resources Group from East Africa by Multiplex SNaPshotTM Analysis of Single Nucleotide Polymorphisms within the Cytochrome Oxidase Subunit I
JR Stothard; J Llewellyn-Hughes; CE Griffin; SJ Hubbard; TK Kristensen & D Rollinson

Abstract

Identification of populations of Bulinus nasutus check for this species in other resources and B. globosus from East Africa is unreliable using characters of the shell. In this paper, a molecular method of identification is presented for each species based on DNA sequence variation within the mitochondrial cytochrome oxidase subunit I (COI) as detected by a novel multiplexed SNaPshotTM assay. In total, snails from 7 localities from coastal Kenya were typed using this assay and variation within shell morphology was compared to reference material from Zanzibar. Four locations were found to contain B. nasutus and 2 locations were found to contain B. globosus. A mixed population containing both B. nasutus and B. globosus was found at Kinango. Morphometric variation between samples was considerable and UPGMA cluster analysis failed to differentiate species. The multiplex SNaPshotTM assay is an important development for more precise methods of identification of B. africanus group snails. The assay could be further broadened for identification of other snail intermediate host species.

Keywords
Bulinus check for this species in other resources - cytochrome oxidase - single nucleotide polymorphism - schistosomiasis - Schistosoma haematobium check for this species in other resources

 
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