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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 61-69
Bioline Code: oc02189
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 61-69

 en Clustering of Schistosoma mansoni check for this species in other resources mRNA Sequences and Analysis of the Most Transcribed Genes: Implications in Metabolism and Biology of Different Developmental Stages
Francisco Prosdocimi; Alessandra C Faria-Campos; Fabiano C Peixoto; Sérgio DJ Pena; José M Ortega & Glória R Franco

Abstract

The study of the Schistosoma mansoni check for this species in other resources genome, one of the etiologic agents of human schistosomiasis, is essential for a better understanding of the biology and development of this parasite. In order to get an overview of all S. mansoni catalogued gene sequences, we performed a clustering analysis of the parasite mRNA sequences available in public databases. This was made using softwares PHRAP and CAP3. The consensus sequences, generated after the alignment of cluster constituent sequences, allowed the identification by database homology searches of the most expressed genes in the worm. We analyzed these genes and looked for a correlation between their high expression and parasite metabolism and biology. We observed that the majority of these genes is related to the maintenance of basic cell functions, encoding genes whose products are related to the cytoskeleton, intracellular transport and energy metabolism. Evidences are presented here that genes for aerobic energy metabolism are expressed in all the developmental stages analyzed. Some of the most expressed genes could not be identified by homology searches and may have some specific functions in the parasite.

Keywords
Schistosoma mansoni check for this species in other resources - bioinformatics - expressed sequences tag - clustering analysis - metabolism

 
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