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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 95-97
Bioline Code: oc02194
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 95-97

 en SHORT COMUNICATION - A Multiplex-PCR Approach to Identification of the Brazilian Intermediate Hosts of Schistosoma mansoni check for this species in other resources
THDA Vidigal; KG Magalhães; JC Kissinger; RL Caldeira; AJG Simpson & OS Carvalho

Abstract

Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria check for this species in other resources , and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni check for this species in other resources from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From the previous sequencing of the ITS2 region, one primer was designed to anchor in the 5.8S conserved region and three other species-specific primers in the 28S region, flanking the ITS2 region. These four primers were simultaneously used in the same reaction (Multiplex-PCR), under high stringency conditions. Amplification of the ITS2 region of Biomphalaria snails produced distinct profiles (between 280 and 350 bp) for B. glabrata , B. tenagophila and B. straminea . The present study demonstrates that Multiplex-PCR of ITS2-DNAr showed to be a promising auxiliary tool for the morphological identification of Biomphalaria snails, the intermediate hosts of S. mansoni .

Keywords
Biomphalaria check for this species in other resources - Multiplex-PCR - internal transcribed spacer 2

 
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