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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 99-104
Bioline Code: oc02195
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 99-104

 en Antigenic Community between Schistosoma mansoni check for this species in other resources and Biomphalaria glabrata check for this species in other resources : on the Search of Candidate Antigens for Vaccines
N Chacón; S Losada; B Noya; B Alarcón de Noya & O Noya

Abstract

We have previously confirmed the presence of common antigens between Schistosoma mansoni check for this species in other resources and its vector, Biomphalaria glabrata check for this species in other resources . Cross-reactive antigens may be important as possible candidates for vaccine and diagnosis of schistosomiasis. Sera from outbred mice immunized with a soluble Biomphalaria glabrata antigen ( SBg A) of non-infected B. glabrata snails recognized molecules of SBg A itself and S. mansoni AWA by Western blot. Recognition of several molecules of the SBg A were inhibited by pre-incubation with AWA (16, 30, 36, 60 and 155 kDa). The only specific molecule of AWA, inhibited by SBg A, was a 120 kDa protein. In order to determine which epitopes of SBg A were glycoproteins, the antigen was treated with sodium metaperiodate and compared with non-treated antigen. Molecules of 140, 60 and 24 kDa in the SBg A appear to be glycoproteins. Possible protective effects of the SBg A were evaluated immunizing outbred mice in two different experiments using Freund's Adjuvant. In the first one (12 mice/group), we obtained a significant level of protection (46%) in the total worm load, with a high variability in worm recovery. In the second experiment (22 mice/group), no significant protection was observed, neither in worm load nor in egg production per female. Our results suggest that SBg A constitutes a rich source of candidate antigens for diagnosis and prophylactic studies.

Keywords
cross reactions - antigens - Biomphalaria check for this species in other resources - Schistosoma check for this species in other resources - vaccine

 
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