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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 97, No. s1, 2002, pp. 115-116
Bioline Code: oc02199
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 97, No. s1, 2002, pp. 115-116

 en Characterization of Sm14 Related Components in Different Helminths by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Western Blotting Analysis
Nilton Thaumaturgo; Mônica Magno Vilar; Ricardo Edelenyi & Miriam Tendler

Abstract

Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica check for this species in other resources infection, in animals previously immunized with the Schistosoma mansoni check for this species in other resources vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei check for this species in other resources , and Taenia saginata check for this species in other resources - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests.

Keywords
Sm14 - Schistosoma mansoni check for this species in other resources - immune crossreaction - fatty acid-binding protein

 
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